DNA RESULT INTERPRETATION!!!

[lvmydex]

Hi my husband and I are new to Dexter's we are up to 1 bull and 3 cows and 2 calves. I just received our first DNA test results a month ago and I haven't a clue what it all means. I had contacted TAMU and asked for some guidance and I was told all I needed to know was under the comment section. This did not make me happy as #1 I did pay for the test and would like to know what it all means. I understand it is probably complicated but I see many on this site talking about heard of origin and I would love to come to understand more about my Dexter's. Can any one point me in the correct direction?? What I am, asking for example what is ETH10 219/221?
What does it mean?? I have searched and all I find is research papers after reading several 200+ page reports I still haven't a clue??. I would appreciate any insight!!! Thanks

[kansasdexters]

Hi lvmydex,

On the parentage genotype report from Texas A&M there are 13 microsatellite markers that are reported. Microsatellite markers are also called STRs (Short Tandem Repeats) or SSRs (Simple Sequential Repeats) and they are short segments of DNA that contain 1-6 base pair (bp) repeat nucleotide motifs, and they tend to occur in non-coding regions of the genome. These regions in a population of animals are highly polymorphic, they present in several different alleles, and this is the basis for their usefulness in animal identification and parentage testing.

An animal can possess only two alleles (two values) of every marker, one of which was inherited from the sire and the other from the dam. So, for each microsatellite marker, you will see two values reported, one that came from the dam and one that came from the sire. If the value is the same for both alleles, then only one value is usually reported.

The microsatellite markers that are used for parentage testing and animal identification are located on different chromosomes. Your example, the ETH10 locus, is found on Bovine chromosome 5. The values given in the test report are the measured base pair lengths of the two alleles found at this locus for the animal that was tested.

If the genotype of the sire and the genotype of the dam are in the database, then the genotype for the animal tested can be compared with those genotypes and either parent can be either qualified (a match) or excluded (no match). If both the sire and dam genotypes match to that of the animal being tested, then the Comment Section of the report will say "Sire Qualified, Dam Qualified".

If the genotypes of the sire and the dam are not in the lab's database, then the genotype for the animal tested cannot be compared to them and the Comment Section of the report will say "Sire Not Typed, Dam Not Typed". The genotype of the animal that has been tested will then be entered into the lab's database and can serve to identify that animal and to be compared with any offspring from that animal that are genotyped in the future and entered into the lab's database.

So, if you have a report that says "Sire Qualified, Dam Not Typed", then you'll know that the sire's genotype is in the database (and it was compared to that of the animal being tested and it matched) and the dam's genotype was not in the database (so it could not be compared to that of the animal being tested).

If you have a report that says "Sire Excluded", then you'll know that the sire (of record) genotype is in the lab's database (and it was compared to that of the animal being tested) and it was not a match. If the sire is excluded, then the pedigree of record does not show the correct sire and additional testing (or additional genotype comparisons) will be needed to determine the correct sire (the sire that matches and is "Qualified").

If you have a report that says, "Dam Excluded", then you'll know that the dam (of record) genotype is in the lab's database (and it was compared to that of the animal being tested) and it was not a match. If the dam is excluded, then the pedigree of record does not show the correct dam and additional testing (or additional genotype comparisons) will be needed to determine the correct dam (the dam that matches and is "Qualified").

In order to do full parentage testing, the DNA genotypes of the sire, the dam, and the animal being tested must all be in the same lab's database. The DNA "genotypes" are the values found for the 13 (or more) microsatellite markers reported for each animal. These panels of values are compared with each other and then the sire and dam are either "Qualified" (matched), or "Excluded" (not matched), or "Not Typed (not in the database). These results are reported in the Comment Section of the test report.

Hope this helps.

Patti

[lvmydex]

THANKS Patti and Genebo the education is very much appreciated....

[Lettie]

We wish to thank you again for the stunning ideas you gave Janet when preparing her post-graduate research plus, most
importantly, for providing every one of the ideas in one blog
post. Provided that we had been aware of your
web-site a year ago, we'd have been saved the useless
measures we were having to take. Thank you very much.

sex shop